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IgG ELISA

redcoon België GENTAUR BVBA

VAT BE0473327336

Av. de l Armee 68 B4

1040 Brussels BELGIUM

  Tel + 32 16 58 90 45 

Fax + 32 16 50 90 45

GENTAUR France SARL

SIRET 48423788800017

Rue Lagrange, 9

75005 Paris, France

 Tel 01 43 25 01 50

Fax 01 43 25 01 60 

GENTAUR Germany

52074 Aachen, Germany

 Tel 0241 40 08 90 86

Fax 0241 55 91 05 36

GENTAUR Pol Sp. Z.o.o. Ulica Ogarna 15/19B m2

80-826 GDANSK

Tel 00 48 51 760 77 08

Fax: 00 32 16 50 90 45

GENTAUR Italy

23015 Milano, Italy

 Tel 02 36 00 65 93

Fax 02 36 00 65 94

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Other Countries redcoon België
0032 (0)16 41 44 07

 

HybridoMax

Hybridoma Growth Supplement

 

 

 

As you may have heard is the FC-210001 ORIGEN Hybridoma Cloning factor no longer available.

 

GENTAUR is pleased to inform you we have a replacement for the FC210001 ORIGEN Hybridoma Cloning factor.

We have an alternative product which enables you to easily prepare your own Serum-Free Hybridoma Growth Medium.

 

The HybridoMax Hybridoma Growth Supplement replaces feeder layers or other conditioned media and growth factors used in hybridoma production. HybridoMax increases the yield of hybridomas surviving HAT selection, the cloning efficiency of B-cell hybridomas, and the number of antibody producing colonies.

 

The HybridoMax Hybridoma Growth Supplement is ready to use. Prepare the serum free Hybridoma growth medium by dissolving 1 bottle of HybridoMax (11 ml) into 1 Liter of D-MEM F-12 (Dulbecco's Modified Eagle's Medium F-12).

 

The prepared Serum-Free Hybridoma growth medium is a chemically defined culture medium supporting the serum-free growth of various hybridomas and 293 cells. The compounds of Hybridoma growth medium include water for injection, a modified DMEM/F12 base, HEPES buffer, known amounts of insulin, transferin, testosterone, sodium selenite, ethanolamine, a variety of saturated and unsaturated fatty acids, and stabilizing proteins. HybridoMax contains no IL-6 and no bovine serum albumine or other undefined protein mixtures.

 

HybridoMax Hybridoma Growth Supplement, 11 ml bottles (for 1 Liter of Hybridoma Growth Medium)

1 bottle of HybridoMax for 57 Euro. (19-HYB-01)

5 bottles of HybridoMax for 270 Euro. (19-HYB-05)

10 bottles of HybridoMax for 510 Euro. (19-HYB-10)

 

You can find the datasheet and the protocol here:

Datasheet.pdf

Protocol.pdf

This protocol can be followed when using the TX-HYB supplemented Hybridoma Growth Medium in combination with a 1000ml cell Bioreactor.

 

We recommend to use the LM-D1223/500 DMEM – Ham’s F12 with L-Glutamine with 15mM HEPES together with the HybridoMax supplement to support optimal hybridoma development and achieve an optimal cell density. These are sold in bottles of 500 ml.

5 bottle of LM-D1223/500 for 59 Euro

10 bottles of LM-D1223/500 for 99 Euro.

20 bottles of LM-D1223/500 for 169 Euro.

 

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